Abstract:

This paper presents an optimized extraction process for gastric mucin from porcine gastric mucosa with aqueous hydrochloric acid solution. A series of single factor experiments were carried out to evaluate the effects of material/liquid ratio, hydrochloric acid concentration, extraction temperature and length of extraction time on gastric mucin yield, and the four process conditions were optimized by quadratic orthogonal rotary combination design based on this. The optimal process conditions for the extraction of gastric mucin were determined as follows: liquid/material ratio, 0.61:1 (mL/g); hydrochloric acid concentration, 5.4%; extraction temperature, 44.9 ℃; and extraction duration 2.0 h. Under these conditions, the yield of gastric mucin was 3.74%, and the product obtained contained 8.7% of total nitrogen and 26.3% of reducing substances and met the quality requirements stipulated by the Ministerial Standards of Chemical Medicine. Thus, the optimized extraction process is feasible for the preparation of gastric mucin.

Key words: gastric mucin, extraction, optimization